Dissertation

Thesis On Biodegradation Of Pesticides

Enhanced degradation of pesticide wastes in soil - Iowa State ... Enhanced degradation of pesticide wastes in soil - Iowa State ...
thesis and dissertation copies are in typewriter face, while others may be from any type .... increase biodegradation of pesticide wastes by soil microorganisms.

Thesis On Biodegradation Of Pesticides

All of these aforementioned problems encouraged the scientific community to develop biological methods to remove endosulfan instead of incineration and landfill methods biodegradation is an efficient bioremediation technique in microorganisms that grow in different ecosystems and through symbiosis with xenobiotics, these microorganisms are able to survive even in incompatible conditions. Most of these microbes live in natural environments, but it is possible to change and strengthen them to decompose pesticides with greater speed. This is an open access article distributed under the terms of the cc-by-nc license (httpcreativecommons.

During the past 50 years, pesticides have been the essential part of the agricultural world. Effect of tween 80 added to the soil on the degradation of endosulfan by kwon g. All of the stations showed significant difference at the 5 level.

The samples were packaged in sterile plastic containers and flasks filled with ice and were transported to the laboratory within 4 hours laboratory chemical manufactured by merck company (darmstadt, germany) were used in this study. Upon degradation of endosulfan stereoisomers, this bacterium produces the metabolites of endosulfan diol 0. When oxidation occurs in a metabolic pathway, endosulfan sulfate is produced.

After transporting the soil samples to the laboratory, bacteria were counted using the total viable plate count method. The extracted samples were analyzed by gas chromatographymass spectrometry (gcms), hp5840 series with a 30-m capillary column (db 1-ms) using a mass-selective detector. Bacterial culture causes rapid degradation of alfa- and beta-endosulfan isomers.

The results showed that bacterial mixed cultures used can be applied to treat soil and water contaminated with endosulfan sp. In addition, various biochemical tests such as the production of acid from carbohydrates, urea breath test, gelatin hydrolysis, indole production, citrate consumption, methyl red, voges proskauer, oxidative-fermentative (of), starch hydrolysis, reduction of nitrate to nitrite, catalase, and oxidase were used the bacteria detected were grown in a mineral medium containing endosulfan (50 gml) for 1 week. The aim of this study was to isolate and identify endosulfan-degrading bacteria from the kor river and also to evaluate the use of bioremediation techniques to improve the environmental status of this river.

This strain quickly degrades endosulfan sulfate and converts it into the less toxic metabolites such as diol, ether, and lactone. In addition, by hydrolysis, both endosulfan diol and endosulfan ether are finally produced furthermore, differences in the degradation of alfa and beta isomers may be due to stereo-isomerization because enzymes released from the bacterial system may correspond to only one of the stereoisomers. Manual of oceanographic and observations pollutant analysis methods (moopam) pp. Degradation of both isomers is associated with the production of metabolites of endosulfan sulfate, endosulfan diol, endosulfan lactone, endosulfan ether, and unknown metabolites in this study, endosulfan diol, endosulfan ether, and endosulfan lactone were metabolites obtained by degradation of alfa- and beta-endosulfan isomers, among which endosulfan diol was the greatest and most original metabolite. The endosulfan were then extracted by solid-phase extraction with methanol.


Biostimulation and enhancement of pesticide degradation around ...


The synopsis part of this thesis is available as a pdf-file for download from the .... the potential for stimulating biodegradation of pesticides (bentazone, mecoprop.

Thesis On Biodegradation Of Pesticides

Biochemical degradation of some pesticides in agricultural soil of ...
Thesis submitted to the Institute of Water and Environment (IWE) ... This work deals with a biochemical degradation of three pesticides: diuron, carbaryl and.
Thesis On Biodegradation Of Pesticides Isolation of bacteria from mechanic workshop soil environment contaminated with used engine oil. Organochlorine pesticides in the surface waters of northern greece. Biodegradation of alpha and beta endosulfan in broth medium and soil microcosm by bacterial strain soto a. The highest count of bacteria in the summer was found in station 3 (8. Endosulfan diol can be converted to endosulfan ether, endosulfan hydroxyl ether, endosulfan dialdehyde, and endosulfan lactone. The initial and remaining values (ppb) of the metabolites were measured. This bacterium brooks majority of endosulfan just after 1 week of incubation. Their results suggested that the metabolism of endosulfan by the cs5 strain was accompanied by a significant reduction in the toxicity.
  • use of fungi in bioremediation of pesticides - Cranfield University


    Average of the logarithm of bacterial counts in three separate stations with endosulfan and without endosulfan in autumn. This is especially true because a concentration of 10 mgl endosulfan is four times greater than its solubility in water. All of these aforementioned problems encouraged the scientific community to develop biological methods to remove endosulfan instead of incineration and landfill methods biodegradation is an efficient bioremediation technique in microorganisms that grow in different ecosystems and through symbiosis with xenobiotics, these microorganisms are able to survive even in incompatible conditions. After 3 weeks of incubation, the bacteria were able to decompose 71. Average frequency of bacterial species isolated from different stations in summer and autumn.

    Pure colonies were obtained by streak cultures several times for identification of endosulfan-degrading bacteria, gram staining, shape of the colony, and movement of bacteria were analyzed. All of the stations showed significant difference at the 5 level. When oxidation occurs in a metabolic pathway, endosulfan sulfate is produced. In addition, various biochemical tests such as the production of acid from carbohydrates, urea breath test, gelatin hydrolysis, indole production, citrate consumption, methyl red, voges proskauer, oxidative-fermentative (of), starch hydrolysis, reduction of nitrate to nitrite, catalase, and oxidase were used the bacteria detected were grown in a mineral medium containing endosulfan (50 gml) for 1 week. Endosulfan is decomposed into endosulfan sulfate by oxidation pathway and into endosulfan diol by hydrolysis.

    Intermediate metabolites known as ii was isolated from soil contaminated with endosulfan. According to the chromatogram obtained from the gcms system, the bacteria with the highest capacity to degrade endosulfan was. The results showed that bacterial mixed cultures used can be applied to treat soil and water contaminated with endosulfan sp. Endosulfan contamination and its persistence in soil and water environments cause it to accumulate in cells of phytoplankton products, zooplankton, fishes, and vegetables. Enrichment and isolation of endosulfan degrading and detoxifying bacteria. The e-screen assay as a tool to identify estrogens an update on estrogenic environmental pollutants. As a result, endosulfan sulfate was not produced during the process of biodegradation in this study, with the predominant biodegradation route being hydrolysis. After transporting the soil samples to the laboratory, bacteria were counted using the total viable plate count method. Despite their benefits, pesticides are compounds that may have toxic side effects, causing potential environmental risk. The aim of this study was to isolate and identify endosulfan-degrading bacteria from the kor river and evaluate the possibility of applying bioremediation in reducing environmental pollution in the desired region.

    Evidence for pesticide degradation in soil by white rot fungi. ...... Figure 1.4 Flow diagram of the experimental work carried out in this thesis............. 36. Figure 2.1 ...

    Fungal degradation of pesticides

    5 Nov 2012 ... This thesis presents the results of my three years as a PhD student at the ..... this PhD were to investigate fungal degradation of pesticides and ...
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    Endosulfan contamination and its persistence in soil and water environments cause it to accumulate in cells of phytoplankton products, zooplankton, fishes, and vegetables. This is an open access article distributed under the terms of the cc-by-nc license (httpcreativecommons. Determination of pesticides in drinking water using solid phase extraction gcms pp. The e-screen assay as a tool to identify estrogens an update on estrogenic environmental pollutants. Regional organization for the protection of the marine environment (ropme) 1999.

    During the biodegradation process, metabolites of endosulfan diol, endosulfan ether, and endosulfan lactone have been produced, all of which have less toxicity than the original compound (i Buy now Thesis On Biodegradation Of Pesticides

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    Endosulfan attaches to gamma-aminobutyric acid receptors located on the membrane of neurons and reduces flow of chloride. The aim of this study was to isolate and identify endosulfan-degrading bacteria from the kor river and evaluate the possibility of applying bioremediation in reducing environmental pollution in the desired region. Thus, in the case of hydrolyzation is the only mechanism of endosulfan degradation. Average of the logarithm of bacterial counts in three separate stations with and without endosulfan in summer. Water and sediment samples were collected from areas with high agricultural activities at three different stations (3 times in each station) in two seasons summer and autumn (fall) Thesis On Biodegradation Of Pesticides Buy now

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    During biodegradation, metabolites of endosulfan diol, endosulfan lactone, and endosulfan ether were also produced, but these had lesser toxicity compared with the original compound (i. Pure colonies were obtained by streak cultures several times for identification of endosulfan-degrading bacteria, gram staining, shape of the colony, and movement of bacteria were analyzed. Samples of surface sediments and water were collected from three different stations in two seasons (summer and autumn), as these are areas with high agricultural activity. Biodegradation and bioremediation of pesticide in soil concept, method and recent developments. Upon degradation of endosulfan stereoisomers, this bacterium produces the metabolites of endosulfan diol 0 Buy Thesis On Biodegradation Of Pesticides at a discount

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    Influence of socioeconomic factors on the pesticides poisoning, brazil. During biodegradation, metabolites of endosulfan diol, endosulfan lactone, and endosulfan ether were also produced, but these had lesser toxicity compared with the original compound (i. Various studies have used endosulfan as a source of sulfur for microbial growth and as a carbon resource in bioremediation. When oxidation occurs in a metabolic pathway, endosulfan sulfate is produced. Intermediate metabolites known as ii was isolated from soil contaminated with endosulfan.

    Isolation of an endosulfan-degrading bacterium from a coffee farm soil persistence and inhibitory effect on its biological functions. The lowest count of bacteria in the autumn was seen in station 1 (6 Buy Online Thesis On Biodegradation Of Pesticides

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    Thus, in the case of hydrolyzation is the only mechanism of endosulfan degradation. The extracted samples were analyzed by gas chromatographymass spectrometry (gcms), hp5840 series with a 30-m capillary column (db 1-ms) using a mass-selective detector. In their study, the appropriate amount of lactone was prepared. Cs5 is able to use endosulfan as a source of carbon, sulfur, and energy. The mixture was then incubated in a shaking incubator at 30c at 150 rpm.

    . Endosulfan diol and endosulfan ether were also produced as the primary metabolites. Endosulfan sulfate is also toxic and stable as the major component (endosulfan). Endosulfan contains two stereoisomers alfa- and beta-endosulfan (ratio, 37) and has been registered under the trade names thiodan, cyclodan, thimol, thiofar, and malix Buy Thesis On Biodegradation Of Pesticides Online at a discount

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    This strain quickly degrades endosulfan sulfate and converts it into the less toxic metabolites such as diol, ether, and lactone. The e-screen assay as a tool to identify estrogens an update on estrogenic environmental pollutants. Production of endosulfan ether has also been reported by other bioremediation studies. Determination of pesticides in drinking water using solid phase extraction gcms pp. Bacterial culture causes rapid degradation of alfa- and beta-endosulfan isomers.

    Once the colonies appear, plates with identifiable and countable colonies were selected and the number of colonies was counted ), deionized water, nutrient agar, and biochemical medium were used in the experiments to enrich endosulfan-degrading bacteria, 5 g of sediment of each station was added to an erlenmeyer flask containing 50 ml of mineral medium and 50 gml of endosulfan pesticide Thesis On Biodegradation Of Pesticides For Sale

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    Effect of tween 80 added to the soil on the degradation of endosulfan by kwon g. Metabolite 1  alfa-endosulfan metabolite 2  beta-endosulfan metabolite 3  endosulfan diol metabolite 4  endosulfan lactone metabolite 5  endosulfan ether. Natural adsorbents such as sediments can control water quality indirectly by absorbing or releasing pesticides. Upon degradation of endosulfan stereoisomers, this bacterium produces the metabolites of endosulfan diol 0. Intermediate metabolites known as ii was isolated from soil contaminated with endosulfan.

    The amounts of degradation of endosulfan isomers and metabolites produced as a result of biodegradation were then analyzed using gas chromatographymass spectrometry For Sale Thesis On Biodegradation Of Pesticides

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    The aim of this study was to isolate and identify endosulfan-degrading bacteria from the kor river and evaluate the possibility of applying bioremediation in reducing environmental pollution in the desired region. Accumulation, metabolism, and effects of organochlorine insecticides on microorganisms. Upon degradation of endosulfan stereoisomers, this bacterium produces the metabolites of endosulfan diol 0. Because of intense agricultural activities in the surrounding kor river, three sampling stations were chosen. The results showed that bacterial mixed cultures used can be applied to treat soil and water contaminated with endosulfan sp.

    These studies have evaluated the ability of various types of fungi and bacteria in biodegradation , degradation of endosulfan and its metabolites was evaluated by gcms analysis Sale Thesis On Biodegradation Of Pesticides

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